Walne’s 培养基 (Walne’s medium)

Walnes 培养基 (Walnes medium)是一种国外常用的海水藻类培养基,光语生物提供商品装培养基,客户也可以根据如下配方配置。

Walne’s Medium Composition

From https://biocyclopedia.com/index/algae/algal_culturing/walnes_medium_composition.php

Reagents Per Liter Seawater
Solution A 1.0 ml
Solution C 0.1 ml
Solution D (to add for diatoms) 2.0 ml
Solution A
FeCl3 * 6H2O 1.3 g
MnCl2 * 4H2O 0.4 g
H3BO3 33.6 g
Na2EDTA 45.0 g
NaH2PO4 * 2H2O 20.0 g
NaNO3 100.0 g
Solution B 1 ml
Solution B per 100ml
ZnCl2 2.1 g
CoCl2 * 6H2O 2.0 g
(NH4)6Mo7O24 * 4H2O 0.9 g
CuSO4 * 5H2O 2.0 g
Concentrated HCl 10 ml
Solution C per 200ml
Thiamine HCl (Vitamin B1) 0.2 g
Cyanocobalamin (Vitamin B12) 10 mg
Solution D (per liter)
Na2SiO3 * 5H2O 40.0 g

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