YBC-II Medium

 

Trichodesmium Medium(束毛藻培养基)

 


NaCl 24.55 g
KCl 0.75 g
NaHCO3 0.21 g
H3BO3 0.036 g
KBr 0.1157
NaF solution (2.94 g/L) 1 ml
MgCl2·6 H2O 4.067 g
MgSO4·7 H2O 6.16 g
CaCl2·2 H2O 1.47 g
SrCl2·6 H2O solution (17.33 g/L) 1 ml
NaH2PO4·H2O solution (6.9 g/L) 1 ml
Trace metals solution 1 ml
Vitamin solution 1 ml
Distilled H2O to 1 L

Filter sterilize. Do not autoclave.

 

Trace metal solution:

Na2EDTA·2 H2O 0.745 g
FeCl3·6 H2O solution (0.11 g/L) 1 ml
MnCl2·4 H2O solution (3.96 g/L) 1 ml
ZnSO4·7 H2O solution (1.15 g/L) 1 ml
Na2MoO4·2 H2O solution (2.66 g/L) 1 ml
CuSO4·5 H2O solution (2.23 g/L) 1 ml
CoCl2·6 H2O solution (5.95 g/L) 1 ml
Distilled water to 1 L

 

Vitamin solution:

Thiamine·HCl 100 mg
Biotin solution (0.5 g/L) 1 ml
Cyanocobalamin solution (0.5 g/L) 1 ml
Distilled water to 1 L

 

Chen, Y.-B., Zehr, J.P. & Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp. IMS 101 in defined media: evidence for a circadian rhythm. J. Phycol. 32: 916-923.

 

ybc-II medium

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