Media for marine and brackish water microalgae

ESM

NaNO3 12 mg

K2HPO4 0.5 mg

Vitamin B12 0.1 µg

Biotin 0.1 µg

Thiamine HCl 10 µg

Fe – EDTA 25.9 µg

Mn – EDTA 33.2 µg

Tris (hydroxymethyl) aminomethane 100 mg

Soil extract 1) 2.5 mL

Seawater 97.5 mL

pH 8.0

Add 1.5 g agar to 100 mL of medium to give a solid medium.

1) The amount of soil extract depends on the quality of the soil. In the NIES-Collection, soil extract was reduced from 5 mL to 2.5 mL after 2002.

Reference

Okaichi, T., Nishio, S., Imatomi, Y. 1982 Collection and mass culture [Shiryô no saisyu to baiyô]. In Toxic phytoplankton – Occurrence, mode of action, and toxins [Yûdoku Purankuton -Hassei, Sayôkikô, Dokuseibun], Ed. by Jpn. Fish. Soc., Kôseisya-Kôseikaku, Tokyo, p. 22-34 (in Japanese without English title).

Soil extract

To 1000 mL distilled water add 200 mL of soil (soil from undisturbed deciduous woodland is best) and heat by autoclaving for 1 h at 105°C. When cool, heat by autoclaving for 1 h at 105°C again. Pass the supernatant through a GF/C filter and Celite, and then pass the filtrate through a GF/F filter. Adjust to 1000 mL by adding distilled water. Dispense 10 mL of the final filtrate into each test tube and sterilize by autoclaving for 20 min at 121°C. Keep in a cool place.

Reference

Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428.