CSI medium
|
# |
组份 |
用量 |
母液浓度 |
|
1 |
Ca(NO3) ·4H2O |
1 mL/L |
15 g/100 mL dH2O |
|
2 |
KNO3 |
1 mL/L |
10g/100 mL dH2O |
|
3 |
MgSO4·7H2O |
1 mL/L |
4g/100 mL dH2O |
|
4 |
β-Na2 glycerophosphate·5H2O |
1 mL/L |
2 .5g/100 mL dH2O |
|
5 |
Vitamin B12 |
0.1ug/L |
|
|
6 |
Biotin |
0.1ug/L |
|
|
7 |
Thiamine HCl |
10ug/L |
|
|
8 |
PIV* |
6ml/L |
|
|
9 |
HEPES |
0.5g/L |
|
|
10 |
Na2SiO3·9H2O |
0.1g/L |
|
|
9 |
土壤提取液** |
30ml/L |
PH调到7.0
*PIV
|
组份 |
浓度 |
|
Na2EDTA |
0.75g/L dH2O |
|
MnCl2·4H2O |
0.041g/L dH2O |
|
ZnCl2·7H2O |
0.005g/L dH2O |
|
Na2MoO4·2H2O |
0.004g/L dH2O |
|
FeCl3·6 H2O |
0.097g/L dH2O |
|
CoCl2.6 H2O |
0.002g/L dH2O |
**土壤提取液
土壤提取液配制方法: 取花园土未施过肥200g置于烧杯或三角瓶中，加入蒸馏水1000毫升，瓶口用透气塞封口，在水浴中沸水加热3小时，冷却，沉淀24小时，此过程连续进行 3次，然后过滤，取上清液，于高压灭菌锅中灭菌后于4℃冰箱中保存备用。
