AF-6 medium
|
# |
组份 |
用量 |
母液浓度 |
|
1 |
NaNO3 |
0.14g |
|
|
2 |
NH4NO3 |
0.22g |
|
|
3 |
K2HPO4 |
0.005g |
|
|
4 |
KH2PO4 |
0.01g |
|
|
5 |
CaCO3 |
0.01g |
|
|
6 |
Citric acid |
0.002g |
|
|
7 |
Biotin |
1ml |
2μg/100ml |
|
8 |
Thiamine HCl |
0.5ml |
1μg/100ml |
|
9 |
Fe-citrate |
0.002g |
|
|
10 |
MgSO4. 7H2O |
0.03 g |
|
|
11 |
CaCl2. 2H2O |
0.01g |
|
|
12 |
PIV* |
5ml |
|
|
13 |
Vitamin B6 |
1μg |
|
|
14 |
Vitamin B12 |
1μg |
|
|
15 |
Distilled water |
995ml |
PH调到6.6
*PIV
|
组份 |
浓度 |
|
Na2EDTA |
0.75g/L dH2O |
|
MnCl2·4H2O |
0.041g/L dH2O |
|
ZnCl2·7H2O |
0.005g/L dH2O |
|
Na2MoO4·2H2O |
0.004g/L dH2O |
|
FeCl3·6 H2O |
0.097g/L dH2O |
|
CoCl2.6 H2O |
0.002g/L dH2O |
af-6培养基怎么配
按照配方配啊，查阅溶解度配置独立的贮备液，使用的时候按照比例混合
这个最后加去离子水的体积都没有写。。。并不知道终体积是多少啊
http://101.96.10.64/mcc.nies.go.jp/medium/en/af6.pdf
请参考这个配方