Guillard and Hargraves (1993) – please see note at the bottom of this page
This enriched seawater medium is based upon f/2 medium (Guillard and Ryther 1962) but has additional trace metals. It is a general purpose marine medium for growing coastal algae.
To prepare, begin with 950 mL of filtered natural seawater. Add the quantity of each component as indicated below, and then bring the final volume to 1 liter using filtered natural seawater. The trace element solution and vitamin solutions are given below. Autoclave. Final pH should be 8.0 to 8.2.
L1 Trace Element Solution
To 950 mL dH2O add the following components and bring final volume to 1 liter with dH2O. Autoclave.
f/2 Vitamin Solution
(Guillard and Ryther 1962, Guillard 1975)
First, prepare primary stock solutions. To prepare final vitamin solution, begin with 950 mL of dH2O, dissolve the thiamine, add the amounts of the primary stocks as indicated in the quantity column below, and bring final volume to 1 liter with dH2O. At the NCMA we autoclave to sterilize. Store in refrigerator or freezer.